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English: Aggregative adherence pattern of EAEC. HEp-2 cells (106) were grown to 50%–70% confluency as monolayers in a 6-well flat-bottom tissue culture plate. After overnight growth cells were washed and 2 mL of fresh DMEM media (pH 7.4) was added, and EAEC grown overnight at 37°C (215 rpm) was inoculated (25 μL) into the plate and incubated at 37°C overnight in 5% CO2 atmosphere. Following incubation, the cells were washed, fixed, and stained with 2.5% Giemsa for 15 minutes. The adherence patterns were examined under 40-X magnification and photographed at 100-X magnification with digital camera in a light microscope. Data are a representative experiment from three independently performed experiments with similar results.