File:Jem2040595f01 (1) (1).png

English: fig1: Spatial and functional CD8+ CTL activity during an acute genital herpetic lesion. (a) Hematoxylin and eosin staining of normal arm (left) and lesional skin (middle). Immunofluorescence staining (right) for CD8+ cells (green) and HSV-2–infected cells (red). (b) Confocal image of CD8+ and HSV-2–infected cells in an acute lesion. Montages of a CD8+ cell (green) in proximity to HSV-2–infected cells (blue) were displayed at 1-μm intervals (left). (middle and right) The 0° (front) and 180° (back) views of a three-dimensional rendering of the interactions. (c) HSV-2–specific CD8 cytolytic activity as measured by chromium release in lymphocytes cultured from a lesion biopsy 5 d after onset, using either bulk or CD8-enriched cultured cells as effectors, and either the autologous or HLA-mismatched B lymphocyte blasts uninfected or HSV-2 infected as targets. Effector/target cell ratio = 20:1. Bars: (a) 50 μm; (b) 10 μm.