File:PMC1084330 pbio.0030156.jpg
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PMC1084330_pbio.0030156.jpg (245 × 246 pixels, file size: 20 KB, MIME type: image/jpeg)
Summary
Description |
English: pbio-0030156-g008: Ultrastructure of H1-HeLa Cells Infected with Human Rhinovirus 14 (HRV14) and Immunoelectron Microscopy of Cells Infected with Poliovirus(A) Cells were infected with rhinovirus 14 as in Figure 3 and prepared for electron microscopy by high-pressure freezing. Examples of readily discernable double lipid bilayers are designated with large arrowheads; vesicles that contain intralumenal viral particles are denoted with small arrows.(B and C) Cells were transfected with a plasmid that expresses GFP-LC3 and subsequently infected with poliovirus for 5 h as in Figure 2. GFP-LC3 was visualized using a secondary antibody coupled to 10-nm gold particles; examples of such particles are denoted with arrows. An arrowhead identifies apparently extracellular packets of cytosol.(D) Cells transfected with an GFP-LC3-expressing plasmid and infected with poliovirus as in (C) were immunostained using an antibody directed against VP1, a viral capsid protein and visualized using a secondary antibody conjugated to 10-nm gold particles; examples of such particles are identified with arrows. |
Date | |
Source | https://openi.nlm.nih.gov/detailedresult?img=PMC1084330_pbio.0030156.g008&query=rhinovirus&it=xg&req=4&npos=1 |
Author | Jackson WT, Giddings TH, Taylor MP, Mulinyawe S, Rabinovitch M, Kopito RR, Kirkegaard K |
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{{subst:Custom license marker added by UW}} https://creativecommons.org/licenses/by/4.0/ Attribution 4.0 International (CC BY 4.0)
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current | 20:38, 25 May 2023 | 245 × 246 (20 KB) | Ozzie10aaaa (talk | contribs) | Uploaded a work by Jackson WT, Giddings TH, Taylor MP, Mulinyawe S, Rabinovitch M, Kopito RR, Kirkegaard K from https://openi.nlm.nih.gov/detailedresult?img=PMC1084330_pbio.0030156.g008&query=rhinovirus&it=xg&req=4&npos=1 with UploadWizard |
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